Cell organization and disturbance-mapping using image activated cell-profiling (CODIAC)

CODIAC addresses the lack of scalable methods for monitoring spatial localization changes of many proteins simultaneously. By coupling image-activated cell sorting (ICS) with improved Cas12a-assisted endogenous PCR tagging and machine-learning-guided gating, it enables disturbance-mapping of cellular organization. This approach could accelerate studies of protein dysregulation in disease and support large-scale phenotypic screens. The preprint is available on bioRxiv, marking an early-stage methodological advance with potential broad applications in cell biology and drug discovery.