Genomic repeats for single-cell molecular recording

Current genomic recording methods typically target only one or a few genomic sites, restricting the amount of information that can be stored and often requiring large cell populations. RGRs overcome this by providing up to 400 repeat copies that can be edited by a single guide RNA, enabling more robust and scalable recording of transient cellular signals. The approach is demonstrated in a preprint on bioRxiv, suggesting it could be broadly applicable for lineage tracing and other single-cell history reconstruction studies. By increasing writing space, RGRs may allow researchers to capture more complex or longer-term biological events at single-cell resolution, with sequencing-based readout.